Composite

Part:BBa_K4385019

Designed by: Min An Zhao   Group: iGEM22_Jilin_China   (2022-10-06)
Revision as of 09:39, 7 October 2022 by Minan (Talk | contribs)


Copper-inducible surface display

This part is composed of CusSR two-component signal transport system, PcusC and OmpC-CBP. When the zinc ion concentration in the environment exceeds a certain concentration, the PcusC promotes the transcription of downstream OmpC-CBP, and realizes the adsorption of zinc ions in the environment. The 6*His Tag was added to this sequence to purify the recombinant protein.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 535
    Illegal XbaI site found at 28
    Illegal SpeI site found at 694
    Illegal PstI site found at 1042
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 535
    Illegal NheI site found at 1410
    Illegal NheI site found at 1433
    Illegal SpeI site found at 694
    Illegal PstI site found at 1042
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 535
    Illegal BglII site found at 243
    Illegal BglII site found at 1545
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 535
    Illegal XbaI site found at 28
    Illegal SpeI site found at 694
    Illegal PstI site found at 1042
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 535
    Illegal XbaI site found at 28
    Illegal SpeI site found at 694
    Illegal PstI site found at 1042
    Illegal AgeI site found at 361
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None