Part:BBa_K4375017
anti-CEA Nanobody Display System
This construction consists of 4 described parts. These are: J23101* (BBa_K4375003), anti-CEA-specific Nanobody, (BBa_K4375006 ), Intimin (BBa_K4375012), Myc (BBa_K4375014). They allow the bacterium to be expelled to the tumour surface, thus allowing its visualisation and colonisation on the tumour surface.
Usage and Biology
This construct is meant for detecting cancer cells that express CEA, carcioembrional antigen by bacterial cells. This device contains two different constructs whose production is under the control of a constitutive strong promoter J23101* (BBa_K4375003). CEA-positive cell-specific attachment is achieved by an anti-CEA-specific Nanobody, (BBa_K4375006 ). The Nanobody displayed on the bacteria outer membrane, by the N-terminal domain of a bacterial adhesion factor, Intimin, described in BBa_K4375012. Cancer cell specific interaction can be detected by two epitope tags: Myc (BBa_K4375014) and anti-E, or via the fluorescence of the policistronicly expressed sfGFP.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 29 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2043
Illegal BamHI site found at 4059 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2064
Illegal NgoMIV site found at 4080
Illegal AgeI site found at 4146
Illegal AgeI site found at 4278
Illegal AgeI site found at 4678 - 1000COMPATIBLE WITH RFC[1000]
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