Part:BBa_K4375016
anti-EGFR Nanobody Display System
This construction consists of 4 described parts. These are: J23101* (BBa_K4375003), anti-EGFR-specific Nanobody: 7D12 (BBa_K4375005), Intimin (BBa_K4375012), Myc (BBa_K4375014). They allow the bacterium to be expelled to the tumour surface, thus allowing its visualisation and colonisation on the tumour surface.
Usage and Biology
This construct is meant for detecting cancer cells expressing EGFR receptors.This construct is expressed by the bacterial cells on their surface and can be used to detect the tumour by attaching to cancer cells. This device contains two different constructs whose production is under the control of a constitutive strong promoter J23101* (BBa_K4375003). EGFR-positive cell-specific attachment is achieved by an anti-EGFR-specific Nanobody, 7D12, (BBa_K4375005). The Nanobody displayed on the bacteria outer membrane, by the N-terminal domain of a bacterial adhesion factor, Intimin, described in BBa_K4375012. Cancer cell specific interaction can be detected by two epitope tags: Myc (BBa_K4375014) and anti-E, or via the fluorercence of the policistronicly expressed sfGFP.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 29 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2029
Illegal BamHI site found at 4045 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2050
Illegal NgoMIV site found at 4066
Illegal AgeI site found at 4124
Illegal AgeI site found at 4256
Illegal AgeI site found at 4640 - 1000COMPATIBLE WITH RFC[1000]
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