Part:BBa_K4375012
Intimin domain for surface display (with anti-E epitope tag)
This protein contains the N-terminal domain of intimin, which is a bacterial adhesion factor, D0 an ig-like protein, and an E epitope tag. Intimin beta-barrel transmembrane domain anchors the construct into the outer membrane of the bacteria.
Usage and Biology
Intimin families are large, secreted polypeptides that contain three functional regions: i) a N-terminal signal peptide, that drives their Sec-dependent translocation across the inner membrane; ii) a β-domain, that is anchored into the outer membrane and comprises a 12-stranded β-barrel with a peptide linker running through the lumen of the β-barrel; and iii) a passenger region, that is secreted to the extracellular milieu. Although their mechanism of secretion remains uncertain, Intimin proteins are translocated into the periplasm and then use the β-barrel assembly machine (BAM) complex for insertion into the outer membrane and translocation of the passenger region to the cell surface. The passenger region is found in the C-terminus. The β-barrel of Intimin proteins is followed by a peptide linker that runs through the lumen connecting its C-terminus to the passenger region.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1983
Illegal BamHI site found at 3999 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2004
Illegal NgoMIV site found at 4020 - 1000COMPATIBLE WITH RFC[1000]
References
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0075126#
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