Part:BBa_K4375005

anti-EGFR Nanobody (7D12)

EGFR receptor-specific Nanobody, namely 7D12. It was optimized to the E. coli’s codon set, because in this way it gives a bigger yield. This Nanobody has the ability to bind to the EGFR receptor and thus can be used to identify cells expressing the EGFR receptor.

Usage and Biology

Epidermal growth factor (EGF) is a 53-amino acid cytokine (6.2 kDa) that is secreted by ectodermic cells, monocytes, kidneys, and duodenal glands. EGF stimulates growth of epidermal and epithelial cells. EGF and at least seven other growth factors and their transmembrane receptor kinases play important roles in cell proliferation, survival, adhesion, migration, and differentiation. The EGF receptor (EGFR) family consists of four transmembrane receptors, including EGFR (HER1/erbB-1), HER2 (erbB-2/neu), HER3 (erbB-3), and HER4 (erbB-4). HER1 as well as HER2 are overexpressed on many solid tumor cells such as breast, non–small-cell lung, head and neck, and colon cancers. Nanobodies are the smallest intact antigen-binding fragments (15 kDa) isolated from heavy-chain camelid antibodies, and they exhibit efficient and specific tumor targeting. One of them is Nanobody 7D12.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 46
  Illegal AgeI site found at 178
  
• 1000
  COMPATIBLE WITH RFC[1000]

References

https://www.ncbi.nlm.nih.gov/books/NBK97357/