Composite

Part:BBa_K4146505

Designed by: Xia Wei   Group: iGEM22_BUCT   (2022-09-16)
Revision as of 06:58, 7 October 2022 by Xiavv (Talk | contribs)


pet-lacI-lac-aro10-adh6-T1

used for coding alcoholdehydrogenase and phenylpyruvate decarboxylase


The standard curve for determination of 2-phenylethanol yield.png

Fig. 2.1 The relationship between tryptophan concentration in culture medium and culture time. The concentration of tryptophan is measured by PDAB chromogenic method.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1526
    Illegal EcoRI site found at 2099
    Illegal EcoRI site found at 2956
    Illegal EcoRI site found at 4994
    Illegal SpeI site found at 2441
    Illegal SpeI site found at 3945
    Illegal PstI site found at 3823
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1526
    Illegal EcoRI site found at 2099
    Illegal EcoRI site found at 2956
    Illegal EcoRI site found at 4994
    Illegal SpeI site found at 2441
    Illegal SpeI site found at 3945
    Illegal PstI site found at 3823
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1526
    Illegal EcoRI site found at 2099
    Illegal EcoRI site found at 2956
    Illegal EcoRI site found at 4994
    Illegal BglII site found at 1541
    Illegal XhoI site found at 2371
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1526
    Illegal EcoRI site found at 2099
    Illegal EcoRI site found at 2956
    Illegal EcoRI site found at 4994
    Illegal SpeI site found at 2441
    Illegal SpeI site found at 3945
    Illegal PstI site found at 3823
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1526
    Illegal EcoRI site found at 2099
    Illegal EcoRI site found at 2956
    Illegal EcoRI site found at 4994
    Illegal SpeI site found at 2441
    Illegal SpeI site found at 3945
    Illegal PstI site found at 3823
    Illegal NgoMIV site found at 1418
    Illegal NgoMIV site found at 6107
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None