Generator
Part:BBa_K274210
Designed by: Siming Ma Group: iGEM09_Cambridge (2009-09-19)
CrtEBIY under constitutive promoter
This Biobrick is created by putting enzyme cassette CrtEBIY (with individual rbs) of Part BBa_K274200 under constitutive promoter R0011.
Enzyme cassette CrtEBIY (with individual rbs) of Part BBa_K274200 converts colourless farnesyl pyrophosphate to orange beta-carotene (via intermediates geranylgeranyl pyroiphosphate, phytoene and lycopene).
Amount of beta-carotene produced can be measured by photospectrometer with absorbance at 455nm (beta-carotene extraction using acetone).
Sequence and Features
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2037
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1573
Illegal NgoMIV site found at 1703
Illegal AgeI site found at 788 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//function/biosynthesis
//function/reporter/pigment
//function/reporter/pigment
None |