Composite

Part:BBa_K4368003:Design

Designed by: Carmen Jimenez Amores, Alonso Molina Calvo, Paola Morales Gonzalez   Group: iGEM22_UMA_MALAGA   (2022-09-26)
Revision as of 22:59, 6 October 2022 by Alonso (Talk | contribs)


pcstA + rbs + bglX + terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1609
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1457
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1367


Design Notes

Consideration should be given to the correct optimization of the sequence for use in different organisms.


Source

BglX is from Escherichia coli str. K-12 substr. MC1655.

References