Coding

Part:BBa_K4378005:Design

Designed by: Sigurður Smári Davíðsson   Group: iGEM22_Uppsala   (2022-10-06)
Revision as of 19:20, 6 October 2022 by Siggi (Talk | contribs)

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TphA2, the α-subunit of TPADO


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 813
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1041
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 409

Design Notes

During the design of this part, two internal EcoRI sites were mutated out in silico prior to synthesis. Additionally this part has been codon optimized for E. coli.

Source

Sequence originates from Burkholderia xenovorans, the sequence published here was adapted from the following publication:

Bains J, Wulff JE, Boulanger MJ. 2012. Investigating Terephthalate Biodegradation: Structural Characterization of a Putative Decarboxylating cis-Dihydrodiol Dehydrogenase. Journal of Molecular Biology 423: 284–293.