Coding
fiatluxC

Part:BBa_K4239001:Design

Designed by: Guillaume FULCONIS   Group: iGEM22_INSA_Lyon1   (2022-10-05)
Revision as of 19:19, 6 October 2022 by Gfulconis (Talk | contribs) (→‎Design Notes)


Enhanced luciferase subunits fiatluxC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1311


Design Notes

Every Igem restriction sites (EcoR1, Xba1, Spe1 and Pst1) were removed from the part.

Source

fiatluxCD is made up of fiatluxC and fiatluxD.

References

Gregor C, Gwosch KC, Sahl SJ, Hell SW. Strongly enhanced bacterial bioluminescence with the ilux operon for single-cell imaging. Proc Natl Acad Sci U S A. 2018 Jan 30;115(5):962-967. doi: 10.1073/pnas.1715946115. Epub 2018 Jan 16. PMID: 29339494; PMCID: PMC5798359.