Generator

Part:BBa_K274120

Designed by: Siming Ma   Group: iGEM09_Cambridge   (2009-09-18)
Revision as of 06:16, 18 September 2009 by Registry (Talk | contribs)

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CrtEBI under pBad promoter

This Biobrick is created by putting enzyme cassette CrtEBI (with individual rbs) of Part BBa_K274100 under arabinose-induced promoter I0500.

Enzyme cassette CrtEBI (with individual rbs) of Part BBa_K274110 converts colourless farnesyl pyrophosphate to red lycopene (via intermediates geranylgeranyl pyroiphosphate and phytoene).

Amount of lycopene produced can be measured by photospectrometer with absorbance at 475nm (lycopene extraction using acetone).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 3192
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2728
    Illegal NgoMIV site found at 2858
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1943
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None