Part:BBa_K4361001
BlcR-binding oligo, 51 bp, wild type
BlcR is a transcription factor originating from the bacterium Agrobacterium tumefaciens (Part:BBa_K4361100). In a homodimer state it contains a single DNA-binding domain that specifically binds one of two DNA sequences. Both sequences are so-called inverted repeat pairs, short DNA sequences whose ends are reverse complements of each other. For the Blc operator, these sequences are 'ACTCTAATgATTCAAGT' and 'ATTAGttgaactCTAAT', as highlighted under "Sequence and Features" below as Inverted repeat pair 1 and Inverted repeat pair 2, respectively. The capitalized nucleotides in each sequence form the inverted repeat pairs, although it should be noted that for the first pair the ends of the sequence are not perfect reverse complements (see Part:BBa_K4361004 and Part:BBa_K4361005).
This original sequence (designated BBa_K4361001) contains two sets of different ‘inverted repeat pairs’, short DNA sequences whose ends are complementary to their own inverse sequence, separated by a 3 nt spacer. Each inverted repeat pair is able to bind a BlcR dimer, with the spacing between pairs allowing for tetramerization of the protein.
This is further described in the wildtype oligo, Part:BBa_K4361001.
-Contains 2 inverted repeats, each binding 1 BlcR dimer
-Kd,app of 120 nM according to EMSA
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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