Composite

Part:BBa_K4385015:Design

Designed by: Min An Zhao   Group: iGEM22_Jilin_China   (2022-10-04)
Revision as of 16:17, 6 October 2022 by Minan (Talk | contribs) (→‎Source)


Copper constitutive surface display


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 414
    Illegal SpeI site found at 573
    Illegal PstI site found at 921
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 414
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1289
    Illegal NheI site found at 1312
    Illegal SpeI site found at 573
    Illegal PstI site found at 921
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 414
    Illegal BglII site found at 122
    Illegal BglII site found at 1424
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 414
    Illegal SpeI site found at 573
    Illegal PstI site found at 921
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 414
    Illegal SpeI site found at 573
    Illegal PstI site found at 921
    Illegal AgeI site found at 240
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It is mainly composed of constitutive promoter(BBa_J23100) , the anchoring protein OmpC-CBP(BBa_K4385003, BBa_K4385005) and Heavy metal detoxification protein( BBa_K4385010).


Source

The basic parts that make up the composite part are all derived from E. coli Genomic DNA.

References