Composite

Part:BBa_K4385015

Designed by: Min An Zhao   Group: iGEM22_Jilin_China   (2022-10-04)
Revision as of 16:12, 6 October 2022 by Minan (Talk | contribs)


Copper constitutive surface display

This part was mainly composed of constitutive promoter J23100, the anchoring protein OmpC-CBP and the heavy metal detoxification protein. Promoter J23100 continuously expressed downstream copper ion surface display proteins to verify the function of outer membrane protein C and lipoprotein of copper ion surface display system. The 6*His Tags were added to this sequence to purify the recombinant protein.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 414
    Illegal SpeI site found at 573
    Illegal PstI site found at 921
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 414
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1289
    Illegal NheI site found at 1312
    Illegal SpeI site found at 573
    Illegal PstI site found at 921
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 414
    Illegal BglII site found at 122
    Illegal BglII site found at 1424
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 414
    Illegal SpeI site found at 573
    Illegal PstI site found at 921
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 414
    Illegal SpeI site found at 573
    Illegal PstI site found at 921
    Illegal AgeI site found at 240
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None