Coding

Part:BBa_K4378001:Design

Designed by: Sigurður Smári Davíðsson   Group: iGEM22_Uppsala   (2022-10-06)
Revision as of 15:41, 6 October 2022 by Siggi (Talk | contribs)

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Engineered PETase from I. sakaiensis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 118
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

During the design of this part, one internal XbaI site was mutated out in silico prior to synthesis. Additionally this part has been codon optimized for E. coli.

Source

Sequence originates from Ideonella sakaiensis, the sequence published here was adapted from the following publication:

Austin HP, Allen MD, Donohoe BS, Rorrer NA, Kearns FL, Silveira RL, Pollard BC, Dominick G, Duman R, El Omari K, Mykhaylyk V, Wagner A, Michener WE, Amore A, Skaf MS, Crowley MF, Thorne AW, Johnson CW, Woodcock HL, McGeehan JE, Beckham GT. 2018. Characterization and engineering of a plastic-degrading aromatic polyesterase. Proceedings of the National Academy of Sciences of the United States of America 115: E4350–E4357.