Protein_Domain
Part:BBa_K4137011:Design
Designed by: YA-JHIH KO Group: iGEM22_GEMS_Taiwan (2022-10-06)
CcdB coding sequence + 6xHis
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 234
Design Notes
The 6xHis tag is used for protein purification and identification.
Source
https://benchling.com/stuberosum/f/lib_5cCjIjmn-kill-switch/seq_G4wUDoDs-final-ccdb-constuct/edit