Regulatory

Part:BBa_K239005:Design

Designed by: Axel Nystrom   Group: iGEM09_UCL_London   (2009-06-26)
Revision as of 11:23, 16 September 2009 by Axelnystrom (Talk | contribs) (→‎References)

NarK promoter, Fnr activated under anaerobic conditions


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Detect and remove possible restriction sites: XbaI, EcoRI, PstI, SpeI, AgeI, BamHI, BglII, NogMIV, NotI and XhoI. No restriction site had to be removed. Sequence starts 3 bp before the IHF binding site and ends 6 pb after the -10 box.


Source

Genomic sequence from biocyc, Escherichia coli K-12. Native unmodified sequence.

References

Regulation of narK gene expression in Escherichia coli in response to anaerobiosis, nitrate, iron, and molybdenum. Kolesnikow T, Schröder I, Gunsalus RP. J Bacteriol. 1992 Nov;174(22):7104-11.


Bonnefoy V, DeMoss JA (1992). "Identification of functional cis-acting sequences involved in regulation of narK gene expression in Escherichia coli." Mol Microbiol 1992;6(23);3595-602. PMID: 1474901