Composite
const. cI

Part:BBa_K4368005

Designed by: Carmen Jimenez Amores, Alonso Molina Calvo, Paola Morales Gonzalez   Group: iGEM22_UMA_MALAGA   (2022-09-26)
Revision as of 14:51, 6 October 2022 by Alonso (Talk | contribs)


pcstA + rbs + cI + terminator

Description

It is a composition based on the cI repressor coding region of bacteriophage lambda, which features an LVA tail for rapid degradation of the protein (BBa_C0051). In the case of our team, it has been placed under the control of the pcstA promoter (BBa_K118011), together with a strong rbs (BBa_B0030) and double terminator (BBa_B0015). This part of our group's collection, iGEM22_UMA_MALAGA, is used as a mechanism of gene regulation, as the transcription and subsequent translation of the repressor protein will be influenced by the concentration of glucose generated in the medium by the transformed bacteria.

Characterization

xxx

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Contribution

  • Group: [1]
  • Author: Jiménez Amores, Carmen


[edit]
Categories
Parameters
None