Composite

Part:BBa_K4204014

Designed by: Chujing Wu   Group: iGEM22_BNDS_China   (2022-10-06)
Revision as of 14:32, 6 October 2022 by WuChujing (Talk | contribs)

Lys+Quality control

Description

The quality control system is added to LysPBC5. Protein quality control systems include a switch at the 5’ end of an mRNA and a corresponding trigger sequence with reverse complementary to the switch at the 3’ end of the mRNA. When the Cis-trigger appears, the switch will bind with the trigger preferentially because the Cis-trigger has a higher affinity with the switch. Or, the switch binds with its complementary sequence and clocks the RBS inside a neck-ring structure; this blocks the transcription initiation. The QC system applied to the LysPBC5 increase the amount of functional protein. Other IGEM teams can use the tested ProQC system for full-length protein expression to increase the amount of functional protein.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 907
    Illegal PstI site found at 31
    Illegal PstI site found at 367
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 907
    Illegal NheI site found at 1017
    Illegal NheI site found at 1099
    Illegal PstI site found at 31
    Illegal PstI site found at 367
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 907
    Illegal BglII site found at 916
    Illegal BamHI site found at 1794
    Illegal XhoI site found at 2122
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 907
    Illegal PstI site found at 31
    Illegal PstI site found at 367
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 907
    Illegal PstI site found at 31
    Illegal PstI site found at 367
    Illegal NgoMIV site found at 346
    Illegal NgoMIV site found at 1846
    Illegal AgeI site found at 496
  • 1000
    COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None