Composite

Part:BBa_K4245001:Design

Designed by: Manasvi Gupta, Natalie Tan, Vandana Vijay Kumar, Christina Cho, Nick Lockhart, Hari Mudigonda, Janet Standeven   Group: iGEM22_Lambert_GA   (2022-08-17)
Revision as of 14:05, 6 October 2022 by NickL (Talk | contribs) (→‎Source)


Spinach aptamer with LacI repression


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 361
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The composite part was acquired from DNA synthesis companies. The part was transformed into standard iGEM high copy plasmids.

Source

The Spinach aptamer was originally generated by the Jaffrey Lab at Cornell University using the SELEX (systematic evolution of ligands by exponential enrichment) process (add reference).

References

Pothoulakis, G., Ceroni, F., Reeve, B., & Ellis, T. (2013). The spinach RNA aptamer as a characterization tool for synthetic biology. ACS Synthetic Biology, 3(3), 182–187. https://doi.org/10.1021/sb400089c