Part:BBa_K4165139

TLKIVW Peptide

Tau binding peptide targeting the PHF seed of Tau

Usage and Biology

TLKIVW is a hexapeptide designed using Rosetta software to bind to VQIVYK steric zipper motif of tau fibers, which is from primarily reasons for the Alzheimer’s disease and other dementia related diseases, also it can prevent further accumulation of tau fibrils.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Dry Lab

Modeling

TLKIVW were modeled by AlphaFold2 and Apptest. The best model obtained from Apptest

                            Figure 1: Predicted 3D structure of Synthetic peptide TLKIVW.

Table 1: Quality assessment parameters of TLKIVW model.

References

1. Sievers, S. A., Karanicolas, J., Chang, H. W., Zhao, A., Jiang, L., Zirafi, O., ... & Eisenberg, D. (2011). Structure-based design of non-natural amino-acid inhibitors of amyloid fibril formation. Nature, 475(7354), 96-100.