Coding

Part:BBa_K4425004

Designed by: Junfei Xia   Group: iGEM22_SUSTech_Shenzhen   (2022-09-30)
Revision as of 09:39, 6 October 2022 by SeanHANPT (Talk | contribs)

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inactive trp repressor

an inactive trp repressor that created by introducing a random sequence into wild type trp repressor

Figure 1: schematic of generating inactive trp repressor


Usage and Biology

inactive trp repressor was used to generate the library of mock selection to confirm the feasibility of CPR cycle in directed evolution of trp repressor

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 540


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Categories
Parameters
None