Composite

Part:BBa_K4368000

Designed by: Carmen Jimenez Amores, Alonso Molina Calvo, Paola Morales Gonzalez   Group: iGEM22_UMA_MALAGA   (2022-09-26)
Revision as of 09:28, 6 October 2022 by Alonso (Talk | contribs)

pcstA + rbs + yebF + cenA + terminator

CenA encodes for the endoglucanase gene of Celullomonas fimi (BBa_K118023). This enzyme is responsible of the degradation of celullose working coordinated with th genes cex and bglX. In addition, this part includes the composition used by the team, which includes a strong rbs (BBa_B0030), a double terminator (BBa_B0015) as well as a promoter inducible by glucose concentration (BBa_K118011). Furthermore, we improve this composite adding a gen encoding a motor protein named YebF (BBa_K1610300) that secrete the enzyme out of the E. coli membrane. The gene has been placed under the control of this promoter to build the glucose concentration-based gene regulatory circuit that integrates all our parts.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 554
    Illegal NotI site found at 1698
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 268
    Illegal BglII site found at 1611
    Illegal BamHI site found at 747
    Illegal XhoI site found at 1109
    Illegal XhoI site found at 1358
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 889
    Illegal NgoMIV site found at 1814
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 793

Contribution

  • Group: [1]
  • Author: Molina Calvo, Alonso


[edit]
Categories
Parameters
None