Composite

Part:BBa_K4463004

Designed by: Qiaowa Gong   Group: iGEM22_XJTLU-CHINA   (2022-09-18)
Revision as of 03:08, 6 October 2022 by Qiaowa (Talk | contribs)


CsgA-SmtA
CsgA is the major curli subunit protein of E.coli. Therefor, the surface display of SmtA, a protein belongs to Metallothionein family, achieved by linking to csgA.

IGEM_XJTLU 2022 verified the function of CsgA-SmtA by measuring the concentration of Cu(II) and Cd(II) adsorbed by SmtA protein on the surface of E.coli.

Figure1. E.coli expressed SmtA protein showed higher adsorption of Cu(II) in 1.8mM CuSO4 solution.

Figure1. E.coli expressed SmtA protein showed higher adsorption of Cd(II) in 300μM CdCl2 solution.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 561
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 561
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 561
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 561
    Illegal AgeI site found at 589
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
Parameters
chassisE.coli