Composite

Part:BBa_K4321007:Design

Designed by: Kulay Janneh   Group: iGEM22_Guelph   (2022-09-28)
Revision as of 00:40, 6 October 2022 by Kjanneh (Talk | contribs) (→‎Source)


Cyt1Aa Cassette


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 264
    Illegal PstI site found at 1006
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 264
    Illegal PstI site found at 1006
    Illegal NotI site found at 1888
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 264
    Illegal BglII site found at 1304
    Illegal BamHI site found at 683
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 264
    Illegal PstI site found at 1006
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 264
    Illegal PstI site found at 1006
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Unlike Cyt2Ba, the stability of the Cyt1Aa protein and colony formation in host and E.coli cells are reliant on the co-expression of a P20 helper protein. When trying to express Cyt1Aa, this factor should be considered.

Source

The cassette was synthesized by IDT. Information on the origin of each part can be found on their respective part pages: P20 helpher protein - BBa_K2938004, Cyt1Aa - BBa_K2938003, RBS - BBa_B0034, mScarlet - BBa_K4321004, Terminator - BBa_K4321008.

References