Part:BBa_K4388004

Vitis vinifera Resveratrol O-Methyltransferase Mutant (VvROMT S29P)

The Vitis vinifera resveratrol o-methyltransferase (VvROMT) is an enzyme that catalyses the dimethylation of resveratrol to form pterostilbene.

ROMT is a key enzyme used for the production of pinostilbene or pterostilbene. This version of the enzyme is a mutant with amino acid change S29P. This mutations has been shown to increase catalytic efficiency from 128 (wild-type) to 158 μmol/h/g (Yan et al., 2021).

The ORF of this compound was obtained from NCBI GenBank code: FM178870.1. This sequence was then codon optimized for E. coli K12, mutated at the desired positions, and two stop codons (TAATAA) were added to terminate translation.

Yan, Z.-B., Liang, J.-L., Niu, F.-X., Shen, Y.-P., & Liu, J.-Z. (2021). Enhanced Production of Pterostilbene in Escherichia coli Through Directed Evolution and Host Strain Engineering. Frontiers in Microbiology, 12. doi:10.1186/s12934-017-0644-6

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 116
  Illegal BamHI site found at 366
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]