Composite

Part:BBa_K4387978

Designed by: Nathalie Weibel   Group: iGEM22_UZurich   (2022-09-30)
Revision as of 17:52, 5 October 2022 by Nathi (Talk | contribs)

Nitric Oxide Sensing Genetic Circuit

This composite part contains the nitric oxide sensor pNorVß, followed by 2 different ribosome binding sites. The promoter induces upon NO binding the expression of a monovalent anti-TNF nanobodoy BBa_K4387996. The additional NorR at the end of the composite part enhances the postitive feedback-loop, increasing the response of the promoter to NO. Together with the hemolysin A secretion machinery BBa_K4387987, a complete genetic circuit is obtained that allows secretion of nanobodies or if exchanged, secretion of other proteins of interest.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 708
    Illegal BglII site found at 1287
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None