Coding
Cyt2Ba

Part:BBa_K4321000:Design

Designed by: Kulay Janneh   Group: iGEM22_Guelph   (2022-09-28)
Revision as of 17:02, 5 October 2022 by Kjanneh (Talk | contribs) (Addition of info)


Cytotoxic Protein 2Ba (Cyt2Ba)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Cyt2Ba forms cytoplasmic inclusion bodies that do no cause cytotoxic effects to the host bacteria. As a result, encoding a helper protein such as P20, that prevent cytotoxic effects on host cells is unnecessary.

Source

Cyt2Ba originates from Bacillus thuringiensis serovar israelensis ISPC-12. The full-length sequence was sourced from Genbank and synthesized as a cassette by IDT.

Reference 1