Part:BBa_K4165077
WAP-four disulphide core domain 10A serine protease inhibitor.
This basic part encodes Human serine protease inhibitor WAP-four disulfide core domain 10A which is able to inhibit HtrA1 (BBa_K4165004).
Usage and Biology
This gene encodes for a type of inhibitor that contains a motif which consists of 8 cysteine residues capable of forming four disulfide bonds at the core of the protease, thus inhibiting its action. This type of inhibitor is very effective and has high affinity for trypsin-like proteases (serine proteases), and in our case it would act as an inhibitor for the trypsin-like catalytic domain of serine protease HtrA1[1]-[3].
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 49
Illegal PstI site found at 198
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 49
Illegal PstI site found at 198
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 49
Illegal PstI site found at 198
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 49
Illegal PstI site found at 198
- 1000COMPATIBLE WITH RFC[1000]
.compatibility div ul,
.compatibility div li {
display: inline;
}
.compatibility div li {
position: relative;
padding-top: 2px;
padding-left:4px;
padding-right:3px;
margin-right:2px;
margin-bottom: 5px;
}
.compatibility div .box {
top: 35px;
width: 200px;
left: 0px;
}
.compatibility div .box white {
border: 1px solid gray;
background-color: white;
}
.compatibility div .box red {
border: 1px solid #dd6666;
background-color: #ffcccc;
background-image: url('https://parts.igem.org/images/red not/red box.png');
background-repeat: none;
}
.compatibility div .box green {
border: 1px solid #44ee44;
background-color: #aaffaa;
}
Assembly Compatibility:
10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 49Illegal PstI site found at 198
12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 49Illegal PstI site found at 198
21COMPATIBLE WITH RFC[21]
23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 49Illegal PstI site found at 198
25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 49Illegal PstI site found at 198
1000COMPATIBLE WITH RFC[1000]
show sequence and features system( 'seq features div', 'BBa K4165077', '0');
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 49
Illegal PstI site found at 198 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 49
Illegal PstI site found at 198 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 49
Illegal PstI site found at 198 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 49
Illegal PstI site found at 198 - 1000COMPATIBLE WITH RFC[1000]
.compatibility div li { display: inline; } .compatibility div li { position: relative; padding-top: 2px; padding-left:4px; padding-right:3px; margin-right:2px; margin-bottom: 5px; } .compatibility div .box { top: 35px; width: 200px; left: 0px; } .compatibility div .box white { border: 1px solid gray; background-color: white; } .compatibility div .box red { border: 1px solid #dd6666; background-color: #ffcccc; background-image: url('https://parts.igem.org/images/red not/red box.png'); background-repeat: none; } .compatibility div .box green { border: 1px solid #44ee44; background-color: #aaffaa; }
Assembly Compatibility:
10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 49Illegal PstI site found at 198 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 49Illegal PstI site found at 198 21COMPATIBLE WITH RFC[21] 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 49Illegal PstI site found at 198 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 49Illegal PstI site found at 198 1000COMPATIBLE WITH RFC[1000]
show sequence and features system( 'seq features div', 'BBa K4165077', '0');Functional Parameters
Isoelectric point (PI) 8.121 Charge at pH 7 4.499 Molecular Weight (Protein) 8.943
Homology and denovo modelling - AlphaFold2 PDB structure https://drive.google.com/drive/folders/1Xzf25fsOO8fyS0w3xAookPZnMpGQ6vrQ Structure assessment results Molprobity = 1.62 Q_Mean = 0.6 土 0.1 Ramachandran Favoured = 89.61% Ramachandran Outliers = 0 Clash Score = 0 C-beta Deviation = 0 Total Score =
References
1. Clauss, A., Lilja, H., & Lundwall, Å. (2005). The evolution of a genetic locus encoding small serine proteinase inhibitors. Biochemical and biophysical research communications, 333(2), 383-389. 2. Eigenbrot, C., Ultsch, M., Lipari, M. T., Moran, P., Lin, S. J., Ganesan, R., ... & Kirchhofer, D. (2012). Structural and functional analysis of HtrA1 and its subdomains. Structure, 20(6), 1040-1050. 3. Grau, S., Baldi, A., Bussani, R., Tian, X., Stefanescu, R., Przybylski, M., ... & Ehrmann, M. (2005). Implications of the serine protease HtrA1 in amyloid precursor protein processing. Proceedings of the National Academy of Sciences, 102(17), 6021-6026.
| None |