Translational_Unit
Part:BBa_K190019:Design
Designed by: Nienke Kuipers Group: iGEM09_Groningen (2009-08-19)
fMT
fMT is a metallothionein, binding Arsenite(III) and Arsenate(V), it has higher affinity for As(III).
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 38
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 127
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Add BioBrick prefix and suffix, also a RBS (B0034) will be added (not in sequence yet)
Source
fMT was cloned from pMAL-MT (kindly provided by Wilfred Chen, University of California), the origin of the gene is the genome of Fucus vesiculosus.
[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Retrieve&db=nucleotide&dopt=GenBank&RID=AJPJ1GVY016&log%24=nuclalign&blast_rank=1&list_uids=3819155 GenBank: AJ005834.1]
References
Highly Selective and Rapid Arsenic Removal by Metabolically Engineered Escherichia coli Cells Expressing Fucus vesiculosus Metallothionein. Singh et al. App and Env Microbiology, May 2008, p. 2924–2927