Translational_Unit

Part:BBa_K190019:Design

Designed by: Nienke Kuipers   Group: iGEM09_Groningen   (2009-08-19)
Revision as of 20:01, 10 September 2009 by Wilfred (Talk | contribs) (→‎Source)

fMT


fMT is a metallothionein, binding Arsenite(III) and Arsenate(V), it has higher affinity for As(III).


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 38
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 127
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Add BioBrick prefix and suffix, also a RBS (B0034) will be added (not in sequence yet)

Source

fMT was cloned from pMAL-MT (kindly provided by Wilfred Chen, University of California), the origin of the gene is the genome of Fucus vesiculosus.

[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Retrieve&db=nucleotide&dopt=GenBank&RID=AJPJ1GVY016&log%24=nuclalign&blast_rank=1&list_uids=3819155 GenBank: AJ005834.1]

References

Highly Selective and Rapid Arsenic Removal by Metabolically Engineered Escherichia coli Cells Expressing Fucus vesiculosus Metallothionein. Singh et al. App and Env Microbiology, May 2008, p. 2924–2927