Coding

Part:BBa_K4197018

Designed by: Guillaume Gomez   Group: iGEM22_Toulouse_INSA-UPS   (2022-09-20)
Revision as of 16:17, 5 October 2022 by Laurelamothe (Talk | contribs)

Gene coding for Gal d 2

Gene coding for the egg allergen called Gal d 2.

Introduction

This part is composed of the gene coding for the allergen of Hen’s egg Gal d 2 (NCBI: V00383.1). The Hen's egg allergy prevalence is 0,5-2,5% in developped countries and Gal d 2 compose 54% of its dry mass (Palosuo and al. 2018). Gal d 2 have already been expressed in Express Iq E. coli and was able to bind the IgE of patient with egg's allergie (Dhanapala and al. 2015).

Construction

The objective of the INSA-UPS 2022 team was to display Gal d 2 on the surface of E. coli so the ordered sequence was merged to OmpA membrane lippoprotein (see part Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 114
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 325


[edit]
Categories
Parameters
None