RNA

Part:BBa_K4345010

Designed by: Paula Ventura   Group: iGEM22_KU_Leuven   (2022-09-29)
Revision as of 10:43, 5 October 2022 by Pventuba (Talk | contribs)


RNA thermometer prfA

RNA thermometer used for post-transcriptional regulation based on a temperature-dependent system, based on a RNA thermometer from Listeria monocytogenes. It is maximally expressed at 37ºC and almost not detectable at 30ºC. It operates as a reversible molecular zipper that controls the availability of the RBS in its structure. When the temperature increases, the thermometer unzips and the RBS can be detected by the ribosome thus allowing translation of downstream mRNA. When the temperature is below a certain range, the RBS gets trapped.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
[edit]
Categories
//regulation/constitutive
//rna/riboswitch
Parameters
biology
control