Protein_Domain

Part:BBa_K4181020

Designed by: Junzhi Yan   Group: iGEM22_SJTU-BioX-Shanghai   (2022-10-05)
Revision as of 08:19, 5 October 2022 by Registry (Talk | contribs)

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cotE→His tag→linker

CotE as an anchor protein can be used to bind melanin to spores. To test whether it is expressed, a His tag is used for verification, followed by a linker to link the subsequent cotB protein.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 298
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 298
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 303
    Illegal XhoI site found at 491
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 298
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 298
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1571


[edit]
Categories
Parameters
None