Coding

Part:BBa_K4244004

Designed by: Arthur Vancolen   Group: iGEM22_Wageningen_UR   (2022-10-04)
Revision as of 06:44, 5 October 2022 by ArthurVancolen (Talk | contribs) (typo)


sfGFP without start codon

The start codon of sfGFP (BBa_K3143689) was removed to allow N-terminus fusion with signal peptide A silent mutation was also performed to removed the SapI site present in BBa_K3143689 at poisition 13 (A to G) to make it compatible with RFC[1000]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None