Composite

Part:BBa_K4385015

Designed by: Min An Zhao   Group: iGEM22_Jilin_China   (2022-10-04)
Revision as of 03:12, 5 October 2022 by Registry (Talk | contribs)

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Copper constitutive surface display

The zinc ion sensor part consists of the ZraSR two-component membrane associated sensor kinase system and the PzraP promoter. ZraSR consists of histidine kinase ZraS, cytoplasmic transcription factor ZraR and periplasmic accessory protein ZraP, responsible for sensing the presence of external Zn2+. The ZraSR system regulates the expression of zraP, which encodes a periplasmic zinc-binding protein, in response to high concentrations of extracellular zinc ions; phosphorylated ZraR triggers divergent transcription of zraP and zraSR.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 414
    Illegal SpeI site found at 573
    Illegal PstI site found at 921
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 414
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1289
    Illegal NheI site found at 1312
    Illegal SpeI site found at 573
    Illegal PstI site found at 921
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 414
    Illegal BglII site found at 122
    Illegal BglII site found at 1424
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 414
    Illegal SpeI site found at 573
    Illegal PstI site found at 921
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 414
    Illegal SpeI site found at 573
    Illegal PstI site found at 921
    Illegal AgeI site found at 240
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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