Composite

Part:BBa_K4167000

Designed by: Xinzichu Xie   Group: iGEM22_ICJFLS   (2022-10-01)
Revision as of 03:06, 5 October 2022 by Yuwang (Talk | contribs)


toehold switch-amilCP

Toehold switch-amilCP was inserted into pSB1C3 which is designed to express amilCP protein triggered by miRNA 34a-5p. It is used to detect the amount of miRNA 34a-5p in samples.

To construct the standard part, toehold switch-amilCP was synthesized and checked the restriction enzyme information, which is showed as follows:

K4167000-fig.1.jpg




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None