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Part:BBa_K4204003

Designed by: Jianfei Song   Group: iGEM22_BNDS_China   (2022-10-04)
Revision as of 02:28, 5 October 2022 by Jianfei Song (Talk | contribs)

Description

This sequence contains 3 protection bases at the 5’ end of the sequence, which is to protect the sequence from damage. And immediately following the T7 promoter, connecting with the sgRNA scaffold, which helps the folding of sgRNA. And at the 3’ end, the target-HBLA region pairs with the targeted B.cereus HBLA gene sequence and enables cleavage.

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