Part:BBa_K2683006

FitD Codon optimized for E.coli

FitD is a protein that is used as molluscicidal toxin. FitD is originally derived from Psuedomonas protegens . This protein has been recognized as a treatment for zebra and quaga mussel populations which are a invasive species in North America[1]. This sequence has been codon optimized for expression in E.coli .

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 2529
  Illegal BglII site found at 2721
  Illegal BamHI site found at 2175
  Illegal BamHI site found at 2277
  Illegal BamHI site found at 2808
  Illegal BamHI site found at 4290
  Illegal BamHI site found at 6462
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 435
  Illegal NgoMIV site found at 1968
  Illegal NgoMIV site found at 2694
  Illegal NgoMIV site found at 2874
  Illegal NgoMIV site found at 2979
  Illegal NgoMIV site found at 3027
  Illegal NgoMIV site found at 4638
  Illegal AgeI site found at 3714
  
• 1000
  COMPATIBLE WITH RFC[1000]

Prairie_iGEM2022_UManitoba

Truncated FitD

This year, we are pleased to add BBa_K4323002to the iGEM registry and hope that it will prove useful to future synthetic biologists. Contrasted with the original iGEM registry FitD sequence BBa_K2683006, the improved part BBa_K4323002is one-third the length, includes a His-tag for simple purification, and is proven to be at least as toxic to Dreissena bugensis as the protein produced by the natural organism.

Figure 1. The toxicity of Truncated FitD to Dreissena bugensis.