Part:BBa_K4368005
pcstA + rbs + cI + terminator
It is a composition based on the cI repressor coding region of bacteriophage lambda, which features an LVA tail for rapid degradation of the protein (BBa_C0051). In the case of our team, it has been placed under the control of the pcstA promoter (BBa_K118011), together with a strong rbs (BBa_B0030) and double terminator (BBa_B0015). This part of our group's collection, iGEM22_UMA_MALAGA, is used as a mechanism of gene regulation, as the transcription and subsequent translation of the repressor protein will be influenced by the concentration of glucose generated in the medium by the transformed bacteria. In the case of high glucose concentration, the pcstA promoter is repressed, preventing the synthesis of the cellulolytic enzymes (controlled under the same promoter), GFP (BBa_K4368006) and the cI protein (BBa_K4368005). In contrast, the glgC16-based construct (BBa_K4368004) would be activated. In contrast, when the glucose concentration is low, the pcstA promoter can transcribe the genes coding for cellulolytic enzymes (BBa_K4368000, BBa_K4368001 and BBa_K4368003) and RFP (BBa_K4368007).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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