Composite

Part:BBa_K4368004

Designed by: Carmen Jimenez Amores, Alonso Molina Calvo, Paola Morales Gonzalez   Group: iGEM22_UMA_MALAGA   (2022-09-26)
Revision as of 16:34, 4 October 2022 by Carmeenjmnezz (Talk | contribs)


pcI + rbs + glgC16 + terminator

GlgC16 encodes for the ADP-glucose pyrophosphorylase gene of Escherichia coli ([1]). This enzyme is responsible for the synthesis of starch-like starch using glucose as a substrate. In addition, this part includes the composition used by the team, which includes a strong rbs (BBa_B0030), a double terminator (BBa_B0015) as well as a promoter inducible by the cI protein of the phage lambda (BBa_R1051). The gene has been placed under the control of this promoter to build the glucose concentration-based gene regulatory circuit that integrates all our parts.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 272
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization from iGEM22_UMA_MALAGA

[edit]
Categories
Parameters
None