Composite
Part:BBa_K4399012
Designed by: Duoqing LIN Group: iGEM22_Worldshaper-Shanghai (2022-09-27)
The inducible anthocyanin biosynthesis system
It is an inducible anthocyanin biosynthesis system, which contains a constitutive GFP expression box, an inducible SbMYB75 expression box, an inducible SbDEL expression box and a constitutive XVE expression box.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1342
Illegal EcoRI site found at 5513
Illegal XbaI site found at 696
Illegal XbaI site found at 1579
Illegal XbaI site found at 8415
Illegal PstI site found at 2759
Illegal PstI site found at 4179
Illegal PstI site found at 4264
Illegal PstI site found at 8599
Illegal PstI site found at 8770 - 1000COMPATIBLE WITH RFC[1000]
Results
The DNA elements (golden gate compatible, promoters: PAtUBI5, PLexA35S, P2×35S; CDS of Genes: GFP, SbMYB75, SbDEL, XVE; Terminators: Tmas, Thsp18.2, Tnos, T35S) were synthesized by Gensript based on their sequences and were sub-cloned into pUC57. The level-0 vectors were then used to contruct Level-1 vectors: pEC47732: PAtUBI5- GFP-Tmas, pEC47742: PLexA35S-SbMYB75-Thsp18.2, pEC47751: PLexA35S-SbDEL-Tnos, pEC47761: P2×35S-XVE-T35S, according to the protocol:
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Categories
Parameters
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