Plasmid

Part:BBa_K4212052:Design

Designed by: Fontaine Gibbs   Group: iGEM22_Imperial_College_London   (2022-09-30)
Revision as of 14:57, 4 October 2022 by Helen737 (Talk | contribs)


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Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 918
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 918
    Illegal NheI site found at 206
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 918
    Illegal BglII site found at 101
    Illegal BglII site found at 261
    Illegal BamHI site found at 903
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 918
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 918
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part consists the chimera of CotZ and ChiS, in which we combined these parts into level 1 Golden Gate Construct. The CotZ is another surface protein located on B.subtilis spores (Liu et al., 2016). Our dry lab team predicted the functional model of this chimera and our wet lab intended to test the effectiveness of this model. We intended to compare the fungal-killing efficiency of CotZ and CotG chimera. tL3S2P21 is a strong promoter, which helps to increase the production of desired protein.


Source

Synthetic construct.

References

[1] Liu, H., Qiao, H., Krajcikova, D., Zhang, Z., Wang, H., Barak, I. & Tang, J. (2016) Physical interaction and assembly of Bacillus subtilis spore coat proteins CotE and CotZ studied by atomic force microscopy. Journal of Structural Biology. 195 (2), 245–251. doi:10.1016/j.jsb.2016.06.010.