![](https://parts.igem.org/images/partbypart/icon_plasmid.png)
Plasmid
Part:BBa_K4212048:Design
Designed by: Yuancheng Ding Group: iGEM22_Imperial_College_London (2022-09-30)
SDP1
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 5222
Illegal XbaI site found at 525
Illegal PstI site found at 1285 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 5222
Illegal NheI site found at 163
Illegal NheI site found at 466
Illegal PstI site found at 1285 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 5222
Illegal BglII site found at 2587 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 5222
Illegal XbaI site found at 525
Illegal PstI site found at 1285 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 5222
Illegal XbaI site found at 525
Illegal PstI site found at 1285
Illegal NgoMIV site found at 441 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 7378
Design Notes
This the final Level 2 Golden Gate assembly for the self-digesting plasmid. The transformed B.subtilis bacteria which successfully take in this construct will digest their own plasmids when germination happens. As a result, the spores produced from transformed B.subtilis will not contain any foreign DNA. This construct ensures the biocontainment.
Source
Synthetic construct