Part:BBa_K4115002:Design

NifA(nif-specific transcriptional activator)

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 136
  Illegal PstI site found at 1000
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 136
  Illegal PstI site found at 1000
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 637
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 136
  Illegal PstI site found at 1000
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 136
  Illegal PstI site found at 1000
  Illegal NgoMIV site found at 1257
  Illegal NgoMIV site found at 1452
  Illegal NgoMIV site found at 1527
  Illegal AgeI site found at 886
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1095
  Illegal SapI site found at 318
  Illegal SapI site found at 939
  

Design Notes

It can be exploited to regulate the expression of nitrogen fixation genes in Azorhizobium caulinodans.

Source

This gene sequence is part of the genome of Azorhizobium caulinodans ORS571. We synthesized this sequence through a commercial synthesis company.

References

[1]Ryu, M. H., Zhang, J., Toth, T., Khokhani, D., Geddes, B. A., Mus, F., Garcia-Costas, A., Peters, J. W., Poole, P. S., Ané, J. M., & Voigt, C. A. (2020). Control of nitrogen fixation in bacteria that associate with cereals. Nature microbiology, 5(2), 314–330. https://doi.org/10.1038/s41564-019-0631-2