Part:BBa_K4244000
ALPaGA Lactate inducible promoter
For the proper functioning of our biosensor construct we must consider that it needs to function in a colonic environment. The lldPRD promoter does not function in the presence of glucose or the absence of oxygen. Unfortunately, both conditions are found in the human colon, meaning that PlldPRD¬ cannot be used to sense lactate in a colonic environment (Schwerdtfeger et al., 2019). Zúñiga et al. developed a lactate sensitive promoter called ALPaGA (A Lactate Promoter Operating in Glucose and Anoxia) (Zúñiga et al., 2021). This promoter is sensitive to lactate in the presence of glucose and absence of oxygen, perfect for developing a biosensor to sense colon cancer. Because we want to use ALPaGA to sense lactate produced by cancer cells, we set out to characterize and compare the native lldPRD operon promoter to ALPaGA. This was done by analysing both promoters in two different conditions: 1) absence of glucose, presence of oxygen and 2) presence of glucose, absence of oxygen, the results of which can be seen in Figure 1 and 2.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
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