Reporter

Part:BBa_K2205000

Designed by: Ansh Vyas   Group: iGEM17_Newcastle   (2017-09-21)
Revision as of 09:26, 4 October 2022 by Svetlana ia (Talk | contribs) (→‎Part Documentation by TU_Dresden 2022 Team)


J23100-RFP

BBa_K2205000 is a proposed composite BioBrick reporter that constitutively produces MFRP1. It possesses an MRFP1 coding sequence (E1010) after a strong Anderson promoter (J23100) and RBS (B0034). A double terminator (B0015) was added after the MRFP1 sequence. A BioBrick RFC10 prefix and suffix were added as well.

The construct was submitted to IDT for synthesis as a gBlock. Due to time constraints, work on assembling and further characterising this Biobrick was unable to finish.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 616
    Illegal AgeI site found at 728
  • 1000
    COMPATIBLE WITH RFC[1000]

Part Documentation by TU_Dresden 2022 Team

Table of content
  1. Biology
  2. Expression
  3. Comparison to constitutative promoter
  4. Item4



  1. Biology
  2. Expression
  3. Comparison to constitutative promoter
  4. Item4

Part was compared to BBa_J04450.

Figure 1: Test description


Usage and Biology

TEst


[edit]
Categories
Parameters
None