Coding

Part:BBa_K4463005

Designed by: Qiaowa Gong   Group: iGEM22_XJTLU-CHINA   (2022-09-18)
Revision as of 08:32, 4 October 2022 by Qiaowa (Talk | contribs)


ShMT3


This is a improved part based on BBa_k4463001. The ability of Metallothioneins to bind metal ions depends on the amount of cysteine (Cys). Therefore, in order to improve the metal ion binding capacity of ShMT, we performed a three-point mutation of the original sequence by targeted mutagenesis to increase the Cys content[1].

Figure1. E.coli expressed ShMT3 protein showed higher adsorption of Cu(II) in 1.8mM CuSO4 solution.
Figure2. E.coli expressed ShMT3 protein showed higher adsorption of Cd(II) in 300μM CdCl2 solution.

Reference

1 X. Li, Z. Ren, M. J. C. Crabbe, L. Wang and W. Ma, Ecotoxicology and Environmental Safety, 2021, 222, 112512.  



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None