Part:BBa_K4271001
T7 Promoter + Lac operator + RBS + OPH + T7 terminator
This sequence is responsible for the regulation and expression of the OPH gene. The T7 promoter, transcribed by only the T7 RNA polymerase, identifies the sequence downstream and enables fast and effective transcription. The Lac operator is a repressor, hence prohibiting the expression of the OPH gene. Therefore, we use IPTG as an inducer to initiate transcription. The ribosome binding site (RBS) is where the ribosome bind on the mRNA for translation. This RBS is taken from the pET22B vector. OPH a gene that encodes organophosphate hydrolase, paraoxon, a type of organic phosphate and insecticide. The product of the paraoxon degradation, pNP, will be detected to evaluate the efficacy of the gene. The T7 terminator identifies the end of the transcription sequence.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1259
Illegal NotI site found at 1219 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1228
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 330
Illegal AgeI site found at 150
Illegal AgeI site found at 435
Illegal AgeI site found at 570
Illegal AgeI site found at 633 - 1000COMPATIBLE WITH RFC[1000]
| None |