Composite
Part:BBa_K4399013
Designed by: Duoqing LIN Group: iGEM22_Worldshaper-Shanghai (2022-09-27)
The inducible anthocyanin biosynthesis system
It is used as a negative control for inducible anthocyanin biosynthesis study, which contains a constitutive GFP expression box, an inducible SbMYB75 expression box and an inducible SbDEL expression box. However, as there is not a XVE box, the activator for PLexA35S, so neither SbMYB75 nor SbDEL can be expressed.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1342
Illegal EcoRI site found at 5513
Illegal XbaI site found at 696
Illegal XbaI site found at 1579
Illegal XbaI site found at 8415
Illegal PstI site found at 2759
Illegal PstI site found at 4179
Illegal PstI site found at 4264
Illegal PstI site found at 8599
Illegal PstI site found at 8770 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1342
Illegal EcoRI site found at 5513
Illegal NheI site found at 1244
Illegal PstI site found at 2759
Illegal PstI site found at 4179
Illegal PstI site found at 4264
Illegal PstI site found at 8599
Illegal PstI site found at 8770 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1342
Illegal EcoRI site found at 5513
Illegal BglII site found at 4113
Illegal BglII site found at 4925
Illegal BglII site found at 6994
Illegal BglII site found at 7321
Illegal BglII site found at 8549
Illegal BamHI site found at 3547
Illegal BamHI site found at 3849
Illegal BamHI site found at 5168
Illegal BamHI site found at 5237
Illegal BamHI site found at 8118
Illegal XhoI site found at 7525 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1342
Illegal EcoRI site found at 5513
Illegal XbaI site found at 696
Illegal XbaI site found at 1579
Illegal XbaI site found at 8415
Illegal PstI site found at 2759
Illegal PstI site found at 4179
Illegal PstI site found at 4264
Illegal PstI site found at 8599
Illegal PstI site found at 8770 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1342
Illegal EcoRI site found at 5513
Illegal XbaI site found at 696
Illegal XbaI site found at 1579
Illegal XbaI site found at 8415
Illegal PstI site found at 2759
Illegal PstI site found at 4179
Illegal PstI site found at 4264
Illegal PstI site found at 8599
Illegal PstI site found at 8770 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
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