Composite

Part:BBa_K4206002

Designed by: Kazuma Tsurumaki   Group: iGEM22_Qdai   (2022-10-03)
Revision as of 04:57, 4 October 2022 by Darjeeling (Talk | contribs)

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cellulases with constitutive promoter

This part contains the coding sequences of endo-1,4-beta-glucanases, exo-1,4-beta-glucanases and beta-glucosidases with PhoA signal sequence. The complete cellulose hydrolysis reaction proceeds by these enzymes in the downstream of constitutive promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 4717
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4350
    Illegal NgoMIV site found at 4723
    Illegal NgoMIV site found at 5225
    Illegal AgeI site found at 378
    Illegal AgeI site found at 1842
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4770
    Illegal SapI.rc site found at 4853


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